The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MCF7 cell lysateLane 2. : 293T cell lysateLane 3. : Jurkat cell lysateLane 4 : SW480 cell lysateLane 5 : PC3 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : 293T cell lysateLane 2. : GPD1L Transfected 293T cell lysate
ICC/IF analysis of GPD1L in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPD1L antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).