Ordering Details
Cat Number:	ATGA0405-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

SOD1/Cu-Zn SOD [AT6F1], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human SOD1 (1-154aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Cu/Zn superoxide dismutase (SOD1), is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. Also over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic,
Ascension Number:	P00441
NCBI EntrezGene:	NP_000445
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : A549 cell lysateLane 2. : A431 cell lysateLane 3. : 293T cell lysateLane 4. : PC3 cell lysateLane 5. : U87MG cell lysate The recombinant protein(20ng) of SOD two isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 (1:1000).Lane 1 : Recombinant protein SOD1Lane 2 : Recombinant protein SOD2

ICC/IF analysis of SOD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SOD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).