The Recombinant Human ADSL (20ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Protein Lane 2. : HeLa cell lysate Lane 3. : 293T cell lysate Lane 4. : Jurkat cell lysate Lane 5. : HepG2 cell lysate Lane 6. : A549 cell lysate Lane 7. : MCF7 cell lysate
ICC/IF analysis of ADSL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADSL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).