The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : PC3 cell lysateLane 2. : U87MG cell lysateLane 3. : WiDr cell lysateLane 4. : Jurkat cell lysateLane 5. : Mouse brain tissue lysate
ICC/IF analysis of ALDOC in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ALDOC in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
