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Cat Number:ATGA0396-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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EMAP-II/AIMP1 [AT6D10], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human AIMP1 (1-336aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:AIMP1, also known as p43, hasn't until recently been recognized for its prominent immunological functions. Together with other nonenzymatic mARS structural components AIMp2/38 and AIMp3/p18, it participates in the machinery responsible for cell-cycle control and tumor suppression.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP2, EMAPII, p43, SCYE1
Ascension Number:Q12904
NCBI EntrezGene:NP_001135888
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIMP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysateLane 3. : Raji cell lysateLane 4. : Jurkat cell lysateLane 5. : A549 cell lysateLane 6. : HaCaT cell lysate

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIMP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysateLane 3. : Raji cell lysateLane 4. : Jurkat cell lysateLane 5. : A549 cell lysateLane 6. : HaCaT cell lysate

ICC/IF analysis of AIMP1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIMP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of AIMP1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AIMP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).