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Cat Number:ATGA0389-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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Arginase 2/ARG2 [AT7F8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human ARG2 (23-354aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Arginase 2 catalyzes the same reaction as arginase 1, but differs in its tissue specificity and subcellular location. Specifically, arginase 2 localizes to the mitochondria. Arginase 2 is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase 1, is not expressed in liver.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase.
Ascension Number:P78540
NCBI EntrezGene:NP_001163
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The SW480 Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : SW480 cell lysate

The SW480 Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : SW480 cell lysate

ICC/IF analysis of ARG2 in SW480 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ARG2 in SW480 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).