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Cat Number:ATGA0387-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
Quantity:
Shipping:£12.00

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LMW-PTP/ACP1 [AT5C11], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human ACP1 (1-158aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Low molecular weight phosphotyrosine protein phosphatase, also known as ACP1, is an enzyme that catalyzes the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. And, It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.
Ascension Number:P24666
NCBI EntrezGene:NP_009030
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3. : TF-1 cell lysate Lane 4. : NIH/3T3 cell lysateLane 5. : HepG2 cell lysate

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3. : TF-1 cell lysate Lane 4. : NIH/3T3 cell lysateLane 5. : HepG2 cell lysate

ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).