Ordering Details
Cat Number:	ATGA0369-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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FUBP1 [AT14F5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant human FUBP1 (279-448aa) purified from E.coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	FUBP1 is a transcriptional regulator and fulfills an important function in the precise control of c-myc transcription. The c-Myc protein is a transcription factor which regulates the transcription of many different target genes that play a role in proliferation, cell cycle progression, differentiation, apoptosis and cell metabolism. Consequently, FUBP1 is also involved in the regulation of proliferation and differentiation, as confirmed by different experimental approaches. Knockdown of FUBP1 or expression of a dominant-negative FUBP1 (DNA-binding domain lacking effector activity) led to proliferation arrest in U2OS and Saos-2 osteosarcoma cells due to reduced c-myc expression.
Further Information:	The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Far upstream element (FuSE) binding protein 1, FBP, FuBP
Ascension Number:	Q96AE4
NCBI EntrezGene:	NP_003893
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUBP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: Jurkat cell lysate

ICC/IF analysis of FUBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FUBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).