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Cat Number:ATGA0365-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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Lactate Dehydrogenase B/LDHB [AT14D7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human LDHB (1-334aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:L-lactate dehydrogenase B chain, LDH-H, TRG-5., L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46
Ascension Number:P07195
NCBI EntrezGene:NP_002291
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant LDHB proteinLane 2. : A549 cell lysateLane 3. : 293T cell lysateLane 4. : K562 cell lysateLane 5. : Jurkat cell lysate

The Recombinant Human LDHB (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant LDHB proteinLane 2. : A549 cell lysateLane 3. : 293T cell lysateLane 4. : K562 cell lysateLane 5. : Jurkat cell lysate

ICC/IF analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).