Ordering Details
Cat Number:	ATGA0362-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

Peroxiredoxin 6 [AT22E7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, FACS
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human PRDX6 (1-224aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Peroxiredoxin 6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell. it can reduce H (2) O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	1-Cys, aiPLA2, AOP2, NSGPx, p29, PRX, Peroxiredoxin6, PRDX6, PRDX 6, PRDX-6, AA690119, Prdx6 rs3, 1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 1 cysPrx, 24 kDa protein, 9430088D19Rik, Acidic calcium independent phospholipase A2, Antioxidant protein 2, Aop2 rs3, Brp 12, mKIAA0106, Ciliary body glutathione peroxidase, CP 3, EC 1.11.1.15, EC 1.11.1.7, EC 3.1.1, GPx, KIAA0106, Liver 2D page spot 40, Ltw4, Lvtw 4, MGC46173, Non selenium glutathione peroxidase, NSGPx, ORF06, OTTHuMP00000032693, Peroxiredoxin6, PHGPx, Phospholipase A2 lysosomal, PLA2, Prdx5, PRX, Red blood cells page spot 12, Thiol specific antioxidant protein,
Ascension Number:	P30041
NCBI EntrezGene:	NP_004896
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant PRDX6 proteinLane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: U87MG cell lysateLane 6.: Ramos cell lysateLane 7.: Mouse brain lysate

ICC/IF analysis of PRDX6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).