| Applications: | ELISA, WB, FACS, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG1 kappa |
| Immunogen: | Recombinant human ACTA2 (3-377aa) purified from E. coli |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic beta- and gamma-actin proteins are referred to as non-muscle actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. Actin has major functions. Firstly, F-actin polymers form microfilaments - polar intracellular tracks for kinesin motor proteins, allowing the transport of vesicles, organelles and other cargo. Actin is a component of the cytoskeleton and links to alpha-actinin, E-cadherin and beta-catenin at adherens junctions. actin has a role in cell motility through polymerization and depolymerization of fibrils. |
| Further Information: | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000. |
| Synonym: | Actin aortic smooth muscle, Actin, aortic smooth muscle, AAT6, ACTSA, MYMY5 |
| Ascension Number: | P62736 |
| NCBI EntrezGene: | NP_001604 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
