Ordering Details
Cat Number:	ATGA0355-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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Beclin 1/BECN1 [AT45E8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant human BECN (1-450aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 is a mammalian ortholog of the yeast autophagy-related gene (Atg) 6 and BEC-1 in the C. elegans nematode. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	beclin 1, ATG6, beclin1, VPS30
Ascension Number:	Q14457
NCBI EntrezGene:	NP_003757
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Recombinant Human BECN1 (10ng) and Cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human BECN1Lane 2.: Balb/3T3 cell lysateThe Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: BECN1 Transfected 293T cell lysate

ICC/IF analysis of BECN1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BECN1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).