Ordering Details
Cat Number:	ATGA0353-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

ACOT8 [AT4D10], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, FACS
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human ACOT8 (1-319aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Acyl-coenzyme A thioesterase 8 is an enzyme that in humans is encoded by the ACOT8 gene. The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene.
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Acyl-coenzyme A thioesterase 8, hACTE-III, HNAACTE, the, PTE-1, PTE-2, PTE1, PTE2
Ascension Number:	O14734
NCBI EntrezGene:	NP_005460
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Recombinant Human ACOT8 (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human ACOT8Lane 2.: Ramos cell lysateLane 3.: A431 cell lysateLane 4 : 293T cell lysateLane 5 : A549 cell lysate

ICC/IF analysis of ACOT8 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT8 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).