Ordering Details
Cat Number:	ATGA0346-50ul
Size:	50uL
Concentration:	1 mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

Glypican 4/GPC4 [AT51E3], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, FACS
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human GPC4 (401-529aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	The Glypican (GPC) family of cell surface heparan sulfate proteoglycans is expressed in a tissue specific and developmentally regulated fashion. Expression of Glypican 4 has been reported in the mouse brain at embryonic day 10 and later stages and low during early tubule formation but is up-regulated in mature tubules. Accordingly it is not detected in the midline of the embryo at the initial stage of neural tube closure, suggesting that GPC4 is unlikely to play an essential role in convergent extension in the mouse.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000, ICC/IF and Flow cytometry is 1:200.
Synonym:	Glypican 4, Glypican 4, dJ900E8.1, glypican proteoglycan 4
Ascension Number:	O75487
NCBI EntrezGene:	NP_001439
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human GPC4Lane 2. : Ramos cell lysate

ICC/IF analysis of GPC4 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPC4 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).