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Cat Number:ATGA0338-50ul
Size:50uL
Concentration:1 mg/mL
Price:£123.00
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Shipping:£12.00

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Cytokeratin 14 [AT13F4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF, IHC
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human KRT14 (1-472aa) purified from E. coli.
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:Keratin 14 (KRT14) is a member of the type I keratin family of intermediate filament proteins. KRT14 gene provides instructions for making a protein called keratin 14. Keratins are a group of tough, fibrous proteins that form the structural framework of certain cells, particularly cells that make up the skin, hair, and nails. Keratin 14 is specifically produced in cells called keratinocytes in the outer layer of the skin.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000
Synonym:Keratin 14, cytokeratin 14, GIG36, MGLAP, NTI
Ascension Number:P02533
NCBI EntrezGene:AAH19097
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Human KRT14 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human KRT14Lane 2.: A431 cell lysate

The Recombinant Human KRT14 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT14 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human KRT14Lane 2.: A431 cell lysate

ICC/IF analysis of KRT14 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human   KRT14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of KRT14 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT14 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).