Ordering Details
Cat Number:	ATGA0336-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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Annexin A1 [AT2G5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human Annexin A1 (1-346aa) purified from E.coli.
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Annexin A1, also known as Lipocortin I, belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. It promotes membrane fusion and is also involved in exocytosis. Since Annexin A1 has phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity, this protein may have potential anti-inflammatory activity. The detection of this protein by immunocytochemical means reportedly provides a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:3000-1:20000.
Synonym:	Annexin-1, Lipocortin I, Calpactin II, Chromobindin-9, p35, ANXA1, ANX1, LPC1, Annexin I, AnnexinA1, AnnexinI, ANX 1, ANX A1, ANXA 1, ANXA1 protein, CalpactinII, Chromobindin 9, Chromobindin9, HGNC:533, Lipocortin 1, Lipocortin I, Lipocortin1, LipocortinI, LPC 1, Phospholipase A2 inhibitory protein,
Ascension Number:	P04083
NCBI EntrezGene:	NP_000691
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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Western blot analysisThe Recombinant Human Annexin A1 (10ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Annexin A1Lane 2.: HeLa cell lysateLane 3.: A549 cell lysateLane 4.: A431 cell lysateThe HeLa cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Annexin A1 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Anti- Annexin A1 antibody (1:10000)Lane 2.: Anti- Annexin A1 antibody (1:20000)