Western blot analysisThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: Ramos cell lysateLane 3.: MCF-7 cell lysateLane 4.: 293T cell lysateLane 5.: HL-60 cell lysateLane 6.: SW480 cell lysateThe Recombinant Human ACAT2 (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: ACAT2 Transfected 293T cell lysate
ICC/IF analysis of ACAT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACAT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
