Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Human Recombinant protein CD3E(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
