Ordering Details
Cat Number:	ATGA0330-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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HABP1/C1QBP [AT1G7], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human C1QBP (74-282aa) purified from E.coli.
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Complement component 1 Q subcomponent-binding protein, mitochondrial is a protein that in humans is encoded by the C1QBP gene. The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:2000.
Synonym:	Complement component 1 q subcomponent binding protein, p33, HABP1, gC1qR, GC1QBP, SF2p32,Complement component 1 q subcomponent binding protein, C1QBP, Complement component 1, q subcomponent binding protein C1q globular domain binding protein, Complement component 1 Q subcomponent binding protein mitochondrial, GC1Q R, GC1q R protein, GC1QR, Glycoprotein gC1qBP, HABP 1, Hyaluronan binding protein 1, Mitochondrial matrix protein p32, p32, Pre mrna splicing factor SF2 P32 subunit precursor, Splicing factor SF2 associated protein.
Ascension Number:	Q07021
NCBI EntrezGene:	NP_001203
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The recombinant protein(50ng) and cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : Recombinant protein Lane 2 : HeLa cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : Ramos cell lysate Lane 6 : 293T cell lysateLane 7 : A549 cell lysate

ICC/IF analysis of C1QBP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human C1QBP antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).