Ordering Details
Cat Number:	ATGA0329-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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Aminoacylase/ACY1 [AT1E2], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant human ACY1 (1-408aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Synonym:	Aminoacylase 1, ACY1D, ACYLASE, Aminoacylase 1
Ascension Number:	Q03154
NCBI EntrezGene:	NP_000657
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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Fig 1.: The cell lysates of Jurkat, HepG2, Hela, A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Recombinant Human ACY1 (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACY1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of ACY1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACY1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).