| Applications: | ELISA, WB |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2b kappa |
| Immunogen: | Recombinant human ASF1B (1-202aa) purified from E. coli |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. Delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA. Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress. |
| Further Information: | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000 |
| Synonym: | ASF1 anti-silencing function 1 homolog B (S.cerevisiae), CIA-II |
| Ascension Number: | Q9NVP2 |
| NCBI EntrezGene: | NP_060624 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
