The cell lysates of HeLa (40ug) and SW480 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSZ antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human CTSBLane 2. : Recombinant Human CTSDLane 3. : Recombinant Human CTSELane 4. : Recombinant Human CTSFLane 5. : Recombinant Human CTSHLane 6. : Recombinant Human CTSKLane 7. : Recombinant Human CTSLLane 8. : Recombinant Human CTSSLane 9. : Recombinant Human CTSWLane 10. : Recombinant Human CTSZ
ICC/IF analysis of CTSZ in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSZ antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).