Ordering Details
Cat Number:	ATGA0278-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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PPM1F [AT6D11], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human PPM1F (1-454aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Synonym:	Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2
Ascension Number:	P49593
NCBI EntrezGene:	NP_055449
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The lysate of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (2ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1F antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: PPM1F Transfected 293T cell lysate

ICC/IF analysis of PPM1F in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1F antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).