Ordering Details
Cat Number:	ATGA0267-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

DAN/NBL1 [AT38G8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human NBL1 (18-181aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	NBL1 is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development and alternatively spliced transcript variants have been identified for this gene. Also, as a tumor suppressor gene of neuroblastoma may play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 have been observed.
Further Information:	The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Synonym:	Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3
Ascension Number:	P41271
NCBI EntrezGene:	NP_005371
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysate of Jurkat (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of NBL1 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NBL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of NBL1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NBL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).