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Cat Number:ATGA0250-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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STAT1 [AT1F4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human STAT1 (1-712aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:STAT1, also known as STAT91, is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:1000. Recommended starting dilution is 1:500
Synonym:Signal transducer and activator of transcription 1 91kDa, ISGF-3, STAT91, Transcription factor ISGF-3 components p91/p84, Signal transducer and activator of transcription 1, 91kDa
Ascension Number:P42224
NCBI EntrezGene:NP_009330
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The HepG2 cell lysate (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STAT1 antibody (1:500, 1:1000, 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The HepG2 cell lysate (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STAT1 antibody (1:500, 1:1000, 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.