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Cat Number:ATGA0248-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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ADI1 [AT27E8], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human ADI1 (1-179aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
Synonym:Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL
Ascension Number:Q9BV57
NCBI EntrezGene:NP_060739.1
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADI1 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADI1 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of ADI1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human      ADI1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ADI1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADI1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).