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Cat Number:ATGA0245-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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ACOT7 [AT1D5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF, FACS
Species Reactivity:Human
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant human ACOT7 (1-370aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2000. Recommended starting dilution is 1:1000.
Synonym:Acyl-CoA thioesterase 7, ACH1, ACT, BACH, CTE-II, hBACH, LACH, LACH1, MGC1126, RP1-120G22
Ascension Number:O00154
NCBI EntrezGene:NP_009205
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The 293T cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The 293T cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACOT7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of ACOT7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ACOT7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACOT7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).