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Cat Number:ATGA0232-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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NAGK [AT4D12], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human NAGK (1-344aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P (II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000.
Synonym:N-acetyl-D-glucosamine kinase, N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase
Ascension Number:Q9UJ70
NCBI EntrezGene:NP_060037.2
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of NAGK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NAGK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of NAGK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NAGK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).