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Cat Number:ATGA0225-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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KRT23 [AT2F6], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human KRT23 (271-422aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:KRT23, also known as cytokeratin 23, is a 422 amino acid intermediate filament protein. The human KRT23 gene is located on chromosome 17q21. 2. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of epithelial cells structure. Cytokeratins are described to be differentiation markers in several epithelial cancers.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:3000.
Synonym:CK-23, CK23, Cytokeratin-23, DKFZP434G032, HAIK1, Cytokeratin 23, MGC26158, K23, K1C23, HAIK 1, Histone deacetylase inducible keratin 23, type I cytoskeletal 23
Ascension Number:Q9C075
NCBI EntrezGene:NP_056330
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of KRT23 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human  KRT23 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of KRT23 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT23 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).