| Applications: | ELISA, WB, ICC/IF, FACS, IHC |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG1 kappa |
| Immunogen: | Recombinant human PNPO (57-261aa) purified from E. coli |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. |
| Further Information: | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000 |
| Synonym: | Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO |
| Ascension Number: | Q9NVS9 |
| NCBI EntrezGene: | NP_060599 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
