The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: Brain tissue lysate Lane 4.: MCF cell lysate
ICC/IF analysis of ARF1 in MCF7 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of ARF1 in U87MG cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
