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Cat Number:ATGA0202-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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CASQ2 [AT4E10], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human CASQ2 (20-399aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:CASQ2 (Calsequestrin 2) protein responsible for holding Calcium together with other two proteins junction and triadin at the sarcoplasmic reticulum renders the hear vulnerable to severe cardiac arrhythmias, known as CPVT (catecholaminergic polymorphic ventricular tachycardia), during physical exercise.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000
Synonym:PDIB2, CASQ2, Calsequestrin 2 (cardiac muscle), Calsequestrin 2 fast twitch cardiac muscle, Calsequestrin cardiac muscle isoform, Calsequestrin fast twitch cardiac muscle, Calsequestrin2, Cardiac calsequestrin 2, CASQ 2, ESTM52, FLJ26321, FLJ93514, PDIB2.
Ascension Number:O14958
NCBI EntrezGene:NP_001223
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The extracts of Mouse muscle (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CASQ2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The extracts of Mouse muscle (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CASQ2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of CASQ2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human  CASQ2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of CASQ2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CASQ2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).