The Cell lysates of HeLa and AGS (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT18 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: A549 cell lysate
Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human KRT18 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:500) with Texas Red (Red).ICC/IF analysis of KRT18 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT18 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
