Ordering Details
Cat Number:	ATGA0185-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

Aurora B [AT2B1], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG2b kappa
Immunogen:	Recombinant human Aurora kinase B (1-344aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells
Further Information:	The antibody has been tested by ELISA and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Immunofluorescence is 1:1000 ~ 2000. Recommended starting dilution is 1:1000
Synonym:	Serine/threonine-protein kinase 12, Aurora-B, AIK2, AIM1, ARK2, AurB, aurkb-sv1, aurkb-sv2, IPL1, STK12, aurkb, AIM1, AuRKB, Aurora 1, STK1, STK5, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora/IPL1 related kinase 2, Serine/theronine kinase 12, Serine/threonine protein kinase 12,
Ascension Number:	Q96GD4
NCBI EntrezGene:	NP_004208
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Aurora kinase B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B

ICC/IF analysis of human HeLa cells stained with Hoechst 3342 (Blue) for chromatin staining and monoclonal anti-human Aurora kinase B antibody (1:2000) with Texas Red (Red).