| Applications: | ELISA, WB, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2b kappa |
| Immunogen: | Recombinant human GAPDH (1-335aa) purified from E.coli |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair. |
| Further Information: | The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000 |
| Synonym: | Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, MGC88685, Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, G3PDH, OCAS, p38 component, Glyceraldehyde 3 phosphate dehydrogenase, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, OCT1 coactivator in S phase, 38-KD component, OTTHuMP00000174431, OTTHuMP00000174432, wu:fb33a10. |
| Ascension Number: | P04406 |
| NCBI EntrezGene: | NP_002037 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
