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Cat Number:ATGA0166-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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AGXT [AT2T4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant AGXT (330-392aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:The AGXT gene provides instructions for making a liver enzyme called alanine-glyoxylate aminotransferase. Inside liver cells, this enzyme is found in peroxisomes, structures that contain many different enzymes used to produce energy and the basic materials important for cellular activities. AGXT converts a compound called glyoxylate to glycine, an amino acid that is a building block for making enzymes and other proteins.
Further Information:The antibody has been tested by ELISA and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Immunofluorescence analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.
Synonym:Serpin B5, Protease inhibitor 5, SERPINB5, alanine-glyoxylate aminotransferase, AGXT1, PH1, AGT, SPT, AGT1, oxalosis I, primary hyperoxaluria type 1, L-alanine: glyoxylate aminotransferase 1, serine:pyruvate aminotransferase, glycolicaciduria
Ascension Number:P21549
NCBI EntrezGene:NP_000021
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Recombinant Protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: AGXT recombinant protein

The Recombinant Protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: AGXT recombinant protein

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). ICC/IF analysis of AGXT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AGXT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). ICC/IF analysis of AGXT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AGXT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).