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Cat Number:ATGA0164-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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FUS2 [AT2F4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, ICC/IF, FACS
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant FUS2 (1-308aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Vertebrate FUS2 genes, which are known to be putative tumor suppressor gene, contain several important domains such as the catalytic N-acetyltransferase (NAT) domain. NAT domain is essential enzymes involved in several sophisticated cellular processes such as N-acetylation, O-acetylatin. NAT enzymes may be involved in susceptibility to cancer including colorectal cancer because of the presence of carcinogenic heterocyclic amines in some cooked foods. FUS2 was physically localized to the cytoplasm. Also, FUS2 showed the actin dependent movement, closely related to the polarization in the budding yeast, Saccharomyces cerevisiae.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot and Immunofluorescence analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.
Synonym:Protein fusion-2, FuS2, FuS-2, NAT6, FuS 2, N acetyltransferase 6, Protein fus 2, Protein fusion 2.
Ascension Number:Q93015
NCBI EntrezGene:NP_036323
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: NIH3T3 cell lysateLane 2.: A431 cell lysateThe Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: FUS2 Transfected 293T cell lysate

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: NIH3T3 cell lysateLane 2.: A431 cell lysateThe Cell lysates (1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: FUS2 Transfected 293T cell lysate

Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).

Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).