Ordering Details
Cat Number:	ATGA0154-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

RNH1 [AT1H23], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant Ribonuclease inhibitor 1(RNH1) (7-461aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	Ribonuclease inhibitor 1 (RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.
Further Information:	The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
Synonym:	ribonuclease/angiogenin inhibitor 1, ribonuclease/angiogenin inhibitor, RAI
Ascension Number:	P13489
NCBI EntrezGene:	NP_002930
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RNH1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Jurkat cell lysate Lane 2. : HepG2 cell lysate

ICC/IF analysis of RNH1 in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RNH1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).