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Cat Number:ATGA0139-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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Cyclophilin F [AT1F5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF, IHC
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human PPIF (30-207aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-transisomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Further Information:The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.
Synonym:Peptidylprolyl isomerase F, Peptidylprolyl isomerase F, CYP3, Cyp-D, PPIF, Peptidylprolyl isomerase F Cyclophilin 3, Cyclophilin D, Cyclophilin F, Cyp D, CYP3, CypD, hCyP3, Peptidylprolyl isomerase F (cyclophilin F), Peptidyl prolyl cis trans isomerase, mitochondral, Peptidylprolyl isomerase F, PPIase, Rotamase.
Ascension Number:P30405
NCBI EntrezGene:NP_005720
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Cell lysates of 293T and HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPIF (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

ICC/IF analysis of PPIF in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human    PPIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of PPIF in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPIF antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).