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Cat Number:ATGA0129-100ul
Size:100uL
Concentration:1mg/mL
Price:£205.00
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Shipping:£12.00

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KLF4 [AT4E6], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human kLF4 (1-170aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:kLF4, which is also known as the gut-enriched kruppel-like factor (GkLF), is a zinc finger-containing transcription factor that belongs to the kruppel-like family of transcription factors. kLF4 plays important roles during the proliferation and differentiation of epithelial cells. Especially, it is found predominantly in gut and has been shown to be expressed during growth arrest. kLF4 is also involved in cell cycle control since expression of kLF4 inhibits DNA synthesis by blocking the G1/S phase of the cell cycle.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
Synonym:krueppel-like factor 4, EZF, GKLF
Ascension Number:O43474
NCBI EntrezGene:NP_004226.3
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: A549 cell lysateLane 2.: HeLa cell lysate

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: A549 cell lysateLane 2.: HeLa cell lysate