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Cat Number:ATGA0127-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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ADRM1 [AT34C2], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human ADRM1 (1-407aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:ADRM1, also known as proteasomal ubiquitin receptor ADRM1, functions as a proteasomal ubiquitin receptor. This protein is an integral plasma membrane protein which promotes cell adhesion. ADRM1 is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Proteasomal ubiquitin receptor ADRM1, ARM1, GP110, Rpn13
Ascension Number:Q16186
NCBI EntrezGene:NP_783163
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : PC3 cell lysateLane 5 : 293T cell lysateLane 6 : HepG2 cell lysateLane 7 : Jurkat cell lysateLane 8 : U87MG cell lysate

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : PC3 cell lysateLane 5 : 293T cell lysateLane 6 : HepG2 cell lysateLane 7 : Jurkat cell lysateLane 8 : U87MG cell lysate

ICC/IF analysis of ADRM1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human   ADRM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of ADRM1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADRM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).