Ordering Details
Cat Number:	ASN0720-100ul
Size:	100uL
Concentration:	1mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

SNAP25 [4E11], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB (Cell lysate)
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human SNAP25 (1-206aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.
Further Information:	The antibody has been tested by ELISA, Western blot, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa.
Ascension Number:	P60880
NCBI EntrezGene:	NP_570824
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: SNAP25 Transfected 293T cell lysate

ICC/IF analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).