| Applications: | ELISA, WB, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2a kappa |
| Immunogen: | Recombinant human PPP1R14A (1-147aa) purified from E. coli |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. |
| Further Information: | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500. |
| Synonym: | Protein phosphatase 1 regulatory (inhibitor) subunit 14A, CPI17, CPI-17, PPP1INL, Protein phosphatase 1, regulatory (inhibitor) subunit 14A, PPP1R14A, Protein phosphatase 1, regulatory (inhibitor) subunit 14A CPI 17, CPI 17 alpha, CPI 17alpha, CPI17, PKC potentiated inhibitory protein of PP1, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Regulatory subunit 14A, |
| Ascension Number: | Q96A00 |
| NCBI EntrezGene: | NP_150281 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
