| Applications: | ELISA, WB, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2b kappa |
| Immunogen: | Recombinant human PP2Calpha (1-382aa) purified from E. coli |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Protein phosphatase 2C (PP2Calpha) is a Mn2+ - or Mg 2+ -dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNk MAPk pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2Calpha, the prototypical member of the PPM family. |
| Further Information: | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250. |
| Synonym: | Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, PDP1, PDP, PPM2C, PP2C alpha, Protein phosphatase 2C, PDP 1, EC 3.1.3.43, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, PPM1A, Protein phosphatase 1A (formerly 2C), ppm1a, FLJ42306, MGC9201, PP2CA, PP2Calpha, PP2C-alpha, protein phosphatase Mg2+/Mn2+ dependent 1A |
| Ascension Number: | P35813 |
| NCBI EntrezGene: | NP_066283 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
