Ordering Details
Cat Number:	APG0714-50ul
Size:	50uL
Concentration:	1mg/mL
Price:	£123.00
Quantity:
Shipping:	£12.00

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MSDS

UCH-L1/PGP95 [3D9], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, ICC/IF, IHC
Species Reactivity:	Human
Isotype:	Mouse IgG2a kappa
Immunogen:	Recombinant human PGP9.5 (1-223aa) purified from E. coli
Purification:	By protein-G affinity chromatography
Conjugation:	Unconjugated
Description:	PGP9. 5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9. 5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9. 5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Further Information:	The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.
Synonym:	ubiquitin carboxyl-terminal esterase L1, uCHL1, PGP9.5, 1-223aa, uCH-L1, EC 3.4.19.12, ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy, Neuron cytoplasmic protein 9.5, Park 5, Parkinson Disease 5, PGP95, uCH L1, Protein gene product 9.5, ubiquitin C terminal esterase L1, ubiquitin C terminal hydrolase (neuron specific), ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase isozyme L1, ubiquitin thiolesterase, ubiquitin thiolesterase L1.
Ascension Number:	P09936
NCBI EntrezGene:	NP_004172
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse Brain lysate Lane 2.: U87-MG cell lysate

Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

ICC/IF analysis of PGP9.5 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGP9.5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).