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All lanes : Anti-ANO7 Antibody (N-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: LNCaP whole cell lysateLane 3: MGC803 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 106 kDaBlocking/Dilution buffer: 5% NFDM/TBST.