All lanes : Anti-GNAO1 Antibody (C-term) at 1:2000 dilutionLane 1: Mouse cerebellum whole lysateLane 2: Mouse brain whole lysateLane 3: Rat brain whole lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.