All lanes : Anti-EDIL3 Antibody (Center) at 1:1000 dilutionLane 1: CCRF-CEM whole cell lysateLane 2: SH-SY5Y whole cell lysateLane 3: MDA-MB-231 whole cell lysateLane 4: U-2OS whole cell lysateLane 5: Rat brain whole lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDaBlocking/Dilution buffer: 5% NFDM/TBST.