All lanes : Anti-CSNK2A1 Antibody (Y255) at 1:2000 dilutionLane 1: C6 whole cell lysatesLane 2: MOLT-4 whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.