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Anti-FAK1 Antibdoy (Y576) at 1:1000 dilution + A431 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 119 kDaBlocking/Dilution buffer: 5% NFDM/TBST.